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KMID : 0377519870120030481
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Chung-Ang Journal of Medicine
1987 Volume.12 No. 3 p.481 ~ p.497
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Experimental Study on the Effect of Glucocorticosteroids on Epidermal Langerhans Cell in the Guinea Pig
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Abstract
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Langerhans cells have been implicated as playing a key role in the processing and presentation of
- t antigens to lymphocytes in contact allergic cell-mediated immune reactions.
" The present study was undertaken to investigate the effect of repeated topical and systemic
administ- ration of glucocorticosteroids on both Langerhans cell densities and immunologic responsiveness to contact k
sensitizers applied to the treated Guinea pig. Hydrocortisone or fluocinonide was administered daily for
t h 10 consecutive days either topically by application to the skin, or systemically by intraperitoneal injection. Langerhans cell density were determined at various times during and after glucocorticosteroids treatment by staining for plasma membrane ATPase activity in epidermal sheet preparations and electron microscopy. The Guinea pig were sensitized and challenged with DNFB after final glucocorticosteroids treatments.
{
Degrees of hypersensitivities were assayed by measuring the increment of ear swelling in tiie DNF3 challenged control and gIucocorticosteroid-treated groups.
^. The results obtained ;were summarized as follows:
1. Hydrocortisone and fluocinonide caused a significant reduction in ATPase-positive Langerhans cell density in dose-related fashion.
2. In electron microscopy the structural alternations of Langerhans cells were cytoplasmic s : elling dilatation of endoplasmic reticulum and perinuclear cisterna, and vacuolar degeneration.
3. In the course of 10--´14 days after cessation of glucocorticosteroids treatment, the density of "Langerhans cells returned to normal values.
4. These glucocorticosteroids induced alternation in Langerhans cell density ivere accompanied by a substantial impairment in inducing hypersensitivity of the skin.
5. The above findings suggested that glucocorticosteroids might exert their inhibitory effects on contact hypersensitivity through altering epidermal Langerhans cell density, thus interfering ,;ith antigenp_-esenting function of these cells.
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KEYWORD
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